Class I/II hybrid inhibitory oligodeoxynucleotide exerts Th1 and Th2 double immunosuppression

FEBS Open Bio. 2012 Nov 12;3:41-5. doi: 10.1016/j.fob.2012.11.002. Print 2013.


We designed class I/II hybrid inhibitory oligodeoxynucleotides (iODNs), called iSG, and found that the sequence 5'-TTAGGG-3', which has a six-base loop head structure, and a 3'-oligo (dG)3-5 tail sequence are important for potent immunosuppressive activity. Interestingly, splenocytes isolated from ovalbumin (OVA)-immunized mice and treated with iSG3 showed suppression of not only interleukin (IL)-6, IL-12p35, IL-12p40, and interferon (IFN) γ mRNA expression, but also IL-4 and IL-13 mRNA expression. Thus, both Th2 and Th1 immune responses can be strongly suppressed by iODNs in splenocytes from allergen-immunized mice, suggesting usefulness in the treatment of diseases induced by over-active immune activation.

Keywords: Class I iODN; Class I/II hybrid iODN; Class II iODN; ELISA, enzyme-linked immunosorbent assay; IFN, interferon; IL, interleukin; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ODN, oligodeoxynucleotide; OVA, ovalbumin; PO, phosphodiester; PS, phosphorothioate; STAT, signal transducer and activator of transcription; TLR, Toll-like receptor; Th1 cell, type 1 helper T cell; Th2 cell, type 2 helper T cell; iODN; immunosuppression.