The crystal structure of the eukaryotic 40S ribosomal subunit in complex with eIF1 and eIF1A

Nat Struct Mol Biol. 2013 Aug;20(8):1015-7. doi: 10.1038/nsmb.2622. Epub 2013 Jul 14.


Eukaryotic translation initiation factors (eIFs) 1A and 1 are central players in the complex process of start-codon recognition. To improve mechanistic understanding of this process, we determined the crystal structure of the 40S ribosomal subunit in complex with eIF1A and eIF1 from Tetrahymena thermophila at a resolution of 3.7 Å. It reveals the positions of the two factors on the 40S and the conformational changes that accompany their binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Crystallization
  • Eukaryotic Initiation Factors / chemistry*
  • Models, Molecular*
  • Molecular Sequence Data
  • Multiprotein Complexes / chemistry*
  • Protein Conformation*
  • Ribosome Subunits, Small, Eukaryotic / chemistry*
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Tetrahymena thermophila / chemistry*


  • Eukaryotic Initiation Factors
  • Multiprotein Complexes

Associated data

  • PDB/4BPE
  • PDB/4BPN
  • PDB/4BPO
  • PDB/4BPP
  • PDB/4BTS