The miniature genome of a carnivorous plant Genlisea aurea contains a low number of genes and short non-coding sequences

BMC Genomics. 2013 Jul 15;14:476. doi: 10.1186/1471-2164-14-476.

Abstract

Background: Genlisea aurea (Lentibulariaceae) is a carnivorous plant with unusually small genome size - 63.6 Mb - one of the smallest known among higher plants. Data on the genome sizes and the phylogeny of Genlisea suggest that this is a derived state within the genus. Thus, G. aurea is an excellent model organism for studying evolutionary mechanisms of genome contraction.

Results: Here we report sequencing and de novo draft assembly of G. aurea genome. The assembly consists of 10,687 contigs of the total length of 43.4 Mb and includes 17,755 complete and partial protein-coding genes. Its comparison with the genome of Mimulus guttatus, another representative of higher core Lamiales clade, reveals striking differences in gene content and length of non-coding regions.

Conclusions: Genome contraction was a complex process, which involved gene loss and reduction of lengths of introns and intergenic regions, but not intron loss. The gene loss is more frequent for the genes that belong to multigenic families indicating that genetic redundancy is an important prerequisite for genome size reduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Evolution
  • Comparative Genomic Hybridization
  • DNA, Intergenic / genetics
  • DNA, Plant / genetics
  • Genome Size*
  • Genome, Plant*
  • Introns
  • Magnoliopsida / genetics*
  • Molecular Sequence Annotation
  • Phylogeny
  • Sequence Analysis, DNA
  • Transcriptome

Substances

  • DNA, Intergenic
  • DNA, Plant