Identification, characterization, and application of a virulence factor (EfEstA) from Enterococcus faecalis

Eunjin Jang; Bum Han Ryu; Hansol Ju; T Doohun Kim

doi:10.1016/j.biortech.2013.06.086

Identification, characterization, and application of a virulence factor (EfEstA) from Enterococcus faecalis

Bioresour Technol. 2013 Sep:143:691-4. doi: 10.1016/j.biortech.2013.06.086. Epub 2013 Jun 28.

Authors

Eunjin Jang¹, Bum Han Ryu, Hansol Ju, T Doohun Kim

Affiliation

¹ Department of Molecular Science and Technology, Graduate School of Interdisciplinary Programs, Ajou University, Suwon 443-749, South Korea.

PMID: 23860564
DOI: 10.1016/j.biortech.2013.06.086

Abstract

Although virulence factors from pathogenic bacteria have been proposed as promising industrial enzymes, characterization and application of these enzymes have been still unexplored. The objective of this study was to identify and characterize virulence factors from pathogenic bacteria for industrial applications. Here, a virulence factor (EfEstA) from Enterococcus faecalis was identified and characterized using SDS-PAGE, enzyme assays, and molecular modeling. In addition, it has been shown that cross-linked enzyme aggregates (CLEAs) of EfEstA exhibited improved stabilities and high recycling activities compared to free EfEstA. These characteristics of EfEstA shed light on the design of new lipase-based systems for industrial applications.

Keywords: CLEAs; EfEstA; Virulence factor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Computational Biology
Electrophoresis, Polyacrylamide Gel
Enterococcus faecalis / pathogenicity*
Models, Molecular
Virulence Factors*

Substances

Virulence Factors