Divergent RNA transcription: a role in promoter unwinding?

Transcription. Jul-Aug 2013;4(4):162-6. doi: 10.4161/trns.25554. Epub 2013 Jul 1.

Abstract

New approaches using biotinylated-psoralen as a probe for investigating DNA structure have revealed new insights into the relationship between DNA supercoiling, transcription and chromatin compaction. We explore a hypothesis that divergent RNA transcription generates negative supercoiling at promoters facilitating initiation complex formation and subsequent promoter clearance.

Keywords: DNA; anti-sense RNA; chromatin; supercoiling; transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatin / genetics
  • Chromatin / metabolism
  • DNA, Superhelical / chemistry
  • DNA, Superhelical / metabolism
  • Histones / metabolism
  • Humans
  • Promoter Regions, Genetic
  • RNA / chemistry
  • RNA / metabolism*
  • RNA Polymerase II / metabolism
  • RNA, Antisense / metabolism
  • Transcription, Genetic

Substances

  • Chromatin
  • DNA, Superhelical
  • Histones
  • RNA, Antisense
  • RNA
  • RNA Polymerase II