Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Mar;71(5):933-48.
doi: 10.1007/s00018-013-1422-1. Epub 2013 Jul 18.

Acyl-CoA Thioesterase 9 (ACOT9) in Mouse May Provide a Novel Link Between Fatty Acid and Amino Acid Metabolism in Mitochondria

Affiliations

Acyl-CoA Thioesterase 9 (ACOT9) in Mouse May Provide a Novel Link Between Fatty Acid and Amino Acid Metabolism in Mitochondria

Veronika Tillander et al. Cell Mol Life Sci. .

Abstract

Acyl-CoA thioesterase (ACOT) activities are found in prokaryotes and in several compartments of eukaryotes where they hydrolyze a wide range of acyl-CoA substrates and thereby regulate intracellular acyl-CoA/CoA/fatty acid levels. ACOT9 is a mitochondrial ACOT with homologous genes found from bacteria to humans and in this study we have carried out an in-depth kinetic characterization of ACOT9 to determine its possible physiological function. ACOT9 showed unusual kinetic properties with activity peaks for short-, medium-, and saturated long-chain acyl-CoAs with highest V max with propionyl-CoA and (iso) butyryl-CoA while K cat/K m was highest with saturated long-chain acyl-CoAs. Further characterization of the short-chain acyl-CoA activity revealed that ACOT9 also hydrolyzes a number of short-chain acyl-CoAs and short-chain methyl-branched CoA esters that suggest a role for ACOT9 in regulation also of amino acid metabolism. In spite of markedly different K ms, ACOT9 can hydrolyze both short- and long-chain acyl-CoAs simultaneously, indicating that ACOT9 may provide a novel regulatory link between fatty acid and amino acid metabolism in mitochondria. Based on similar acyl-CoA chain-length specificities of recombinant ACOT9 and ACOT activity in mouse brown adipose tissue and kidney mitochondria, we conclude that ACOT9 is the major mitochondrial ACOT hydrolyzing saturated C2-C20-CoA in these tissues. Finally, ACOT9 activity is strongly regulated by NADH and CoA, suggesting that mitochondrial metabolic state regulates the function of ACOT9.

Similar articles

See all similar articles

Cited by 11 articles

See all "Cited by" articles

References

    1. J Biol Chem. 1979 Jun 10;254(11):4516-23 - PubMed
    1. Prog Lipid Res. 2010 Oct;49(4):366-77 - PubMed
    1. J Biol Chem. 1992 Aug 5;267(22):15681-6 - PubMed
    1. BMC Bioinformatics. 2004 Aug 12;5:109 - PubMed
    1. FEBS Lett. 1996 Jun 10;388(1):80-4 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback