Histopathological and Clinical Evaluation of Kombucha Tea and Nitrofurazone on Cutaneous Full-Thickness Wounds Healing in Rats: An Experimental Study

Diagn Pathol. 2013 Jul 17;8:120. doi: 10.1186/1746-1596-8-120.


Background: Kombucha, a fermented tea (KT) is claimed to possess many beneficial properties. The aim of this study was to evaluate clinical and histopathological alterations of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rat.

Methods: In present study 24 Wister -albino rats weighing 150-200 g were selected and divided to two treatment groups as Nitrofurazone ointment (0.2%) and Kombucha tea. Subsequently, the anesthesia was exerted by Ketamin hydrochloride 10% (40 mg/kg) and Xylasine (2 mg/kg) through intra muscular (IM) route. Furthermore, upon preparation of dorsal region of the animal for surgery, a piece of full-thickness skin removed (2 × 2 cm). In order to comparing wounds healing clinically and histologically, once every four days from the commencement, the wounds were photographed and the healed surface was measured by Scion image software.

Result: The clinical findings indicated that the Kombucha fungus resulted in precipitating healing than Nitrofurazone; however, it was not significant (p > 0.05). In order to pathological comparing of wound healing process, several wound biopsies were taken on 4, 8, 12, 16 and 20th days. Additionally, the histopathological results demonstrated that there was inflammation in Nitrofurazone group through twelveth day, somehow the epithelium was formed and abundant vessels were visible. Although on 16th day and the previous days the healing condition of Kombucha fungus was considered as minimal rate, revealing it is similar to Nitrofurazone group on 20th day.

Conclusions: To wrap up. These observations suggest that the Kombucha fungus healing quality was rapid from 12th day to the end of the research, whereas no significant difference was observed.

Virtual slide: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1107407136102196.

Publication types

  • Comparative Study
  • Retracted Publication

MeSH terms

  • Administration, Cutaneous
  • Animals
  • Biopsy
  • Camellia sinensis / microbiology*
  • Disease Models, Animal
  • Female
  • Fermentation
  • Male
  • Nitrofurazone / administration & dosage
  • Nitrofurazone / pharmacology*
  • Ointments
  • Phytotherapy
  • Plants, Medicinal
  • Rats
  • Rats, Wistar
  • Skin / drug effects*
  • Skin / injuries
  • Skin / microbiology*
  • Skin / pathology
  • Tea / microbiology*
  • Time Factors
  • Wound Healing / drug effects*
  • Wounds, Penetrating / drug therapy*
  • Wounds, Penetrating / microbiology*
  • Wounds, Penetrating / pathology


  • Ointments
  • Tea
  • Nitrofurazone