VE-cadherin status as an indicator of microvascular permeability

Methods Mol Biol. 2013;1046:335-42. doi: 10.1007/978-1-62703-538-5_20.

Abstract

VE-cadherin phosphorylation and binding partner status are important indicators of endothelial permeability. Here we describe several techniques aimed at discerning total tyrosine phosphorylation levels of VE-cadherin, VE-cadherin phosphorylation on specific tyrosine residues, and the ability of VE-cadherin to bind its binding partner beta-catenin. Taken together, these approaches to studying VE-cadherin status on microvascular endothelial cells are excellent complements to traditional permeability assays.

MeSH terms

  • Antigens, CD / genetics
  • Antigens, CD / metabolism*
  • Cadherins / genetics
  • Cadherins / metabolism*
  • Capillary Permeability*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism
  • Humans
  • Molecular Biology / methods*
  • Phosphorylation
  • Protein Binding
  • Tyrosine / metabolism
  • beta Catenin / metabolism

Substances

  • Antigens, CD
  • Cadherins
  • beta Catenin
  • cadherin 5
  • Tyrosine