Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

Sci Rep. 2013;3:2234. doi: 10.1038/srep02234.

Abstract

Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Biomarkers / blood
  • Biosensing Techniques* / instrumentation
  • Biosensing Techniques* / standards
  • Blood Proteins*
  • Dose-Response Relationship, Radiation
  • Female
  • Male
  • Membrane Proteins / blood
  • Mice
  • Nanotechnology*
  • Radiation, Ionizing*
  • Radiometry*
  • Reproducibility of Results
  • Serum Amyloid A Protein
  • Time Factors

Substances

  • Biomarkers
  • Blood Proteins
  • Membrane Proteins
  • Saa2 protein, mouse
  • Serum Amyloid A Protein
  • flt3 ligand protein