Development of buffers for fast semidry transfer of proteins

Anal Biochem. 2013 Oct 15;441(2):182-4. doi: 10.1016/j.ab.2013.07.009. Epub 2013 Jul 16.

Abstract

Western blot is an extensively used method for protein detection in cell biology. To optimize this procedure, here we examined a panel of buffers for their ability to efficiently transfer proteins from SDS-polyacrylamide gels onto nitrocellulose membranes in a short 12-min period, designated here as fast semidry transfer. Our results show for the first time that HEPES- and HEPPS/EPPS-based buffers represent the most efficient buffers for fast semidry transfer.

Keywords: HEPES; HEPPS; Protein transfer; Western blot.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Buffers
  • Collodion / chemistry*
  • Electrophoresis, Polyacrylamide Gel / economics
  • Electrophoresis, Polyacrylamide Gel / methods*
  • HEPES / chemistry
  • Membranes, Artificial*
  • Proteins / isolation & purification*
  • Time Factors

Substances

  • Buffers
  • Membranes, Artificial
  • Proteins
  • Collodion
  • HEPES