Identifying differential alternative splicing events from RNA sequencing data using RNASeq-MATS

Methods Mol Biol. 2013;1038:171-9. doi: 10.1007/978-1-62703-514-9_10.

Abstract

RNA sequencing (RNA-Seq) has emerged as a powerful and increasingly cost-effective technology for analysis of transcriptomes. RNA-Seq has several significant advantages over gene expression microarrays, including its high sensitivity and accuracy, broad dynamic range, nucleotide-level resolution, ability to detect novel mRNA transcripts, and ability to analyze pre-mRNA alternative splicing. A major application of RNA-Seq is to detect differential alternative splicing, i.e., differences in exon splicing patterns among different biological conditions. We recently developed a statistical method multivariate analysis of transcript splicing (MATS) for detecting differential alternative splicing events from RNA-Seq data. Here, we describe a computational pipeline RNASeq-MATS based on the MATS algorithm. This pipeline automatically detects and analyzes differential alternative splicing events corresponding to all major types of alternative splicing patterns from RNA-Seq data.

MeSH terms

  • Algorithms
  • Alternative Splicing*
  • Animals
  • Gene Expression Profiling / methods
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • RNA Precursors / genetics*
  • RNA, Messenger / genetics*
  • Sequence Analysis, RNA / methods*

Substances

  • RNA Precursors
  • RNA, Messenger