In this study, the dissipation of pirimiphos-methyl during wheat fermentation by Lactobacillus plantarum was investigated. Sample preparation for GC/MS detection of pirimiphos-methyl residues from fermented wheat substrate was carried out by two steps: extraction with 25 mL of methanol : acetone = 1 : 1 solvent mix for 30 min, followed by clean-up procedure through a glass column with florisil coupled with elution by 25 mL of ethyl acetate : acetone = 4 : 1. To obtain the highest pesticide degradation level, the fermentation conditions were optimized according to response surface methodology. Our results showed that L. plantarum was able to reduce the level of pirimiphos-methyl in wheat. Although pirimiphos-methyl was partially labile during sterilization prior inoculation (~37-50%), and there was also spontaneous chemical degradation of pesticide (~6-11%), overall L. plantarum enhanced degradation from 15 to 34%, that is, to nearly 81%. Additionally, the effect of pirimiphos-methyl on the lactobacilli growth, and efficiency of fermentation, was studied where pirimiphos-methyl inhibit the growth of bacteria in concentrations higher than 5 mg kg(-1), while the presence of pirimiphos-methyl did not overall affect the lactic acid fermentation.
Keywords: degradation; fermentation; lactobacilli; pesticide; wheat.
© 2013 The Society for Applied Microbiology.