Osteoinductive and Osteopromotive Variability among Different Demineralized Bone Allografts

Clin Implant Dent Relat Res. 2015 Jun;17(3):533-42. doi: 10.1111/cid.12118. Epub 2013 Jul 24.


Objective: The purpose of this investigation was to compare the osteoinductive and osteopromotive potential of two widely used demineralized freeze-dried bone allografts (DFDBA) (Osteotech® DFDBA and LifeNet® DFDBA).

Material and methods: Twenty-seven male Wistar rats (mean body weight 200 g) were treated with either DFDBA from Osteotech and LifeNet or control for femoral and intramuscular defects and assigned to histological analysis at 2, 4, and 8 weeks postimplantation. Hematoxylin and eosin (H&E), Safranin-O, tartrate-resistant acid phosphatase (TRAP), and osteopontin (OPN) staining were performed. Quantitative analysis of mineralized new bone to total volume (BV/TV) was assessed by micro-computed tomography.

Results: Both allografts demonstrated osteoinductive potential at 2 weeks as assessed by intramuscular bone formation. LifeNet DFDBA displayed continual new bone formation at 4 and 8 weeks, whereas Osteotech particles were fully resorbed by 4 weeks postimplantation. Femur defects demonstrated significantly greater BV/TV at 4 and 8 weeks with higher expression of OPN staining around LifeNet DFDBA particles. TRAP-positive cells were visible in and around both allograft materials.

Conclusion: The results from the present study indicate that variability among allografts exists. In the present, LifeNet DFDBA supported more new bone formation. Further larger animal models or clinical trials are required to validate these findings.

Keywords: LifeNet; Osteotech; bone formation; demineralized freeze-dried bone allograft (DFDBA); osteoinduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allografts
  • Animals
  • Bone Demineralization Technique
  • Bone Substitutes*
  • Bone Transplantation / methods*
  • Femur / surgery
  • Freeze Drying
  • Immunoenzyme Techniques
  • Male
  • Osteogenesis / physiology*
  • Rats
  • Rats, Wistar
  • X-Ray Microtomography


  • Bone Substitutes