Strategy for analysing the co-operativity of intramolecular interactions in peptides and proteins

J Mol Biol. 1990 Aug 5;214(3):613-7. doi: 10.1016/0022-2836(90)90275-Q.


Double mutant cycles enable the measurement of pairwise interactions in proteins. This method is extended for mutations at any number of positions in the protein. This provides a way for determining the context dependence of pairwise interactions on other neighbouring residues.

MeSH terms

  • Mutation*
  • Peptides / metabolism*
  • Protein Conformation
  • Proteins / metabolism*
  • Thermodynamics


  • Peptides
  • Proteins