Rapid Detection of Carbapenemase-Producing Enterobacteriaceae From Blood Cultures

Clin Microbiol Infect. 2014 Apr;20(4):340-4. doi: 10.1111/1469-0691.12318. Epub 2013 Jul 24.

Abstract

The biochemical-based Carba NP test has been evaluated to detect carbapenemase-producing Enterobacteriaceae (n = 193) directly from spiked blood cultures. It was able to rapidly detect KPC (n = 50), IMP (n = 27), VIM (n = 37), NDM (n = 33) and OXA-48-like producers (n = 46) with sensitivity and specificity of 97.9% and 100%, respectively. This cost-effective technique may be implemented in any microbiology laboratory and offers a reliable test for an early identification of carbapenemase-producing Enterobacteriaceae directly from blood culture that could be useful for the management of infected patients.

Keywords: Carba NP test; Carbapenem; clinical sample.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteremia / microbiology*
  • Bacterial Proteins / analysis*
  • Bacteriological Techniques / economics
  • Bacteriological Techniques / methods*
  • Blood / microbiology*
  • Cost-Benefit Analysis
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / isolation & purification*
  • Enterobacteriaceae Infections / microbiology*
  • Humans
  • Sensitivity and Specificity
  • beta-Lactamases / analysis*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase