Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N Geyer; Nancy D Hanson

doi:10.1016/j.diagmicrobio.2013.06.015

Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Diagn Microbiol Infect Dis. 2013 Oct;77(2):113-7. doi: 10.1016/j.diagmicrobio.2013.06.015. Epub 2013 Jul 23.

Authors

Chelsie N Geyer¹, Nancy D Hanson

Affiliation

¹ Creighton University School of Medicine, Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology (C.R.A.B), 2500 California Plaza, Omaha, NE 68178, USA.

PMID: 23891223
DOI: 10.1016/j.diagmicrobio.2013.06.015

Abstract

A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

Keywords: Beta-lactamase genes; Diagnostic PCR; Rapid amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Electrophoresis, Agar Gel
Gram-Negative Bacteria / enzymology
Gram-Negative Bacteria / genetics*
Gram-Negative Bacteria / isolation & purification
Gram-Negative Bacterial Infections / microbiology*
Humans
Molecular Typing / methods*
Polymerase Chain Reaction / methods*
beta-Lactamases / genetics

Substances

Bacterial Proteins
DNA, Bacterial
beta-Lactamases