Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Diagn Microbiol Infect Dis. 2013 Oct;77(2):143-9. doi: 10.1016/j.diagmicrobio.2013.06.023. Epub 2013 Jul 24.


This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.

Keywords: Multilocus sequence analysis; Multilocus sequence typing; Mycobacterium abscessus; Mycobacterium massiliense; rpoB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Multilocus Sequence Typing / methods*
  • Multilocus Sequence Typing / standards*
  • Mycobacterium / classification*
  • Mycobacterium / genetics
  • Mycobacterium Infections / microbiology*
  • Retrospective Studies


  • Bacterial Proteins
  • DNA, Bacterial