Quantitative Analysis of RASSF1A Promoter Methylation in Hepatocellular Carcinoma and Its Prognostic Implications

Biochem Biophys Res Commun. 2013 Aug 23;438(2):324-8. doi: 10.1016/j.bbrc.2013.07.070. Epub 2013 Jul 24.

Abstract

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide and is caused by the accumulation of genetic and epigenetic alterations in regulatory genes. In this study, we used methylight to detect the methylation status of the RASSF1A promoter in 87 paired HCC samples and analysed the relationship between methylation status and clinicopathological parameters, including prognosis after surgery. We found that the methylation level of the RASSF1A promoter in HCC tissues was significantly higher than that in the corresponding non-tumorous tissues (p<0.0001). Furthermore, the methylation level of the RASSF1A gene promoter in HCC samples was higher in patients with a tumor size ≥ 6cm (p=0.0149) and in patients younger than 50 years old (p=0.0175). However, hypermethylation of the RASSF1A promoter in HCC tissues did not affect the overall survival of patients (p=0.611). Thus, RASSF1A promoter hypermethylation may not be a useful biomarker for the prognosis of HCC.

Keywords: DNA methylation; HCC; Methylight; Prognostic biomarker; RASSF1A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / metabolism*
  • Carcinoma, Hepatocellular / surgery
  • Cell Survival
  • DNA Methylation*
  • Epigenesis, Genetic
  • Female
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / metabolism*
  • Liver Neoplasms / surgery
  • Male
  • Middle Aged
  • Prognosis
  • Promoter Regions, Genetic*
  • Tumor Suppressor Proteins / genetics*
  • Tumor Suppressor Proteins / metabolism

Substances

  • Biomarkers, Tumor
  • RASSF1 protein, human
  • Tumor Suppressor Proteins