Variants in BET1L and TNRC6B associate with increasing fibroid volume and fibroid type among European Americans

Hum Genet. 2013 Dec;132(12):1361-9. doi: 10.1007/s00439-013-1340-1. Epub 2013 Jul 28.

Abstract

Uterine fibroids (UFs) affect 77 % of women by menopause and account for $9.4 billion in yearly healthcare costs. We recently replicated findings from the first UF genome-wide association study (GWAS), conducted in the Japanese. Here we tested these GWAS-discovered SNPs for association with UF characteristics to further assess whether risk varies by sub-phenotypes of UFs. Women were enrolled in Right from the Start (RFTS) and the BioVU DNA repository (BioVU). UF status was determined by pelvic imaging. We tested the top GWAS-associated SNPs for association with UF characteristics (RFTS: type, number, volume; BioVU: type) using covariate adjusted logistic and linear regression. We also combined association results of UF type using meta-analysis. 456 European American (EA) cases and 1,549 controls were examined. Trinucleotide repeat containing 6B (TNRC6B) rs12484776 associated with volume in RFTS (β = 0.40, 95 % CI 0.05-0.75, p = 0.024). RFTS analyses evaluating stratified quartiles of volume showed the strongest OR at rs12484776 for the largest volume (16.6-179.1 cc, odds ratio (OR) = 2.19, 95 % confidence interval (CI) 1.07-4.46, p = 0.031). Meta-analysis showed a strong association at blocked early in transport 1 homolog (BET1L) rs2280543 for intramural UFs (meta-OR = 0.51, standard error (SE) = 0.14, Q = 0.590, I = 0, p = 2.48 × 10(-6)), which is stronger than the overall association with UF risk. This study is the first to evaluate these SNPs for association with UF characteristics and suggests these genes associate with increasing UF volume and protection from intramural UF in EAs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Case-Control Studies
  • European Continental Ancestry Group / genetics*
  • Female
  • Genetic Predisposition to Disease / ethnology
  • Genome-Wide Association Study / statistics & numerical data
  • Humans
  • Leiomyoma / classification
  • Leiomyoma / ethnology
  • Leiomyoma / genetics*
  • Leiomyoma / pathology
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Qc-SNARE Proteins / genetics*
  • RNA-Binding Proteins / genetics*
  • Tumor Burden / genetics*
  • United States
  • Uterine Neoplasms / classification
  • Uterine Neoplasms / ethnology
  • Uterine Neoplasms / genetics*
  • Uterine Neoplasms / pathology
  • Young Adult

Substances

  • BET1L protein, human
  • Qc-SNARE Proteins
  • RNA-Binding Proteins
  • TNRC6B protein, human