RNA-guided gene activation by CRISPR-Cas9-based transcription factors

Nat Methods. 2013 Oct;10(10):973-6. doi: 10.1038/nmeth.2600. Epub 2013 Jul 25.

Abstract

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Clustered Regularly Interspaced Short Palindromic Repeats*
  • HEK293 Cells
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Interleukin 1 Receptor Antagonist Protein / genetics
  • Protein Engineering / methods*
  • RNA Editing*
  • RNA, Guide / genetics*
  • Ribonucleases / genetics
  • Transcription Factors / genetics*
  • Transcriptional Activation*

Substances

  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • RNA, Guide
  • Transcription Factors
  • Ribonucleases

Associated data

  • GEO/GSE47114