Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes

PLoS One. 2013 Jul 23;8(7):e68824. doi: 10.1371/journal.pone.0068824. Print 2013.


Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition / genetics*
  • Genome, Bacterial*
  • Genomics*
  • High-Throughput Nucleotide Sequencing*
  • Lichens / genetics
  • Lichens / microbiology*
  • Streptomyces / genetics*
  • Streptomyces / isolation & purification

Grant support

This work was supported by a Functional Genomics on Polar Organisms grant (PE13020) funded by the Korea Polar Research Institute (KOPRI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.