It has become clear that food crushing is performed in the circumscribed region between functional cusps, that is, at "the main occluding area". However, the position of the main occluding area in patients with fixed partial dentures (FPDs) has not been investigated quantitatively yet. There is a possibility that the load onto the abutment teeth of FPDs is loaded excessively depending on the position of the main occluding area at the early stage of mastication. Therefore the position of the main occluding areas of the FPDs was investigated. The purpose of this study was to evaluate the position of the main occluding area in patients with FPDs quantitatively. We compared the position of the main occluding area between the control subjects who had complete dentition and the patients who wore FPDs for replacing missing first lower molars. Pieces of temporary filling material were used as test material to decide the position of the main occluding area. The centroid coordinate of the test material was calculated and projected onto the reference axis in order to evaluate the position of the main occluding area. Moreover, we recorded the occlusal contact areas at the intercuspal position in order to evaluate the relationship between the position of main occluding area and occlusal contacts.As a result, the position of the main occluding areas in the FPD group were significantly posterior to those in the control group and tended to be less stable. Besides occlusal contact area, existence of periodontal ligaments in the pontic region may cause the difference of the positions of the main occluding area between the FPD group and the control group.