The combination of resveratrol and conjugated linoleic acid attenuates the individual effects of these molecules on triacylglycerol metabolism in adipose tissue

Eur J Nutr. 2014;53(2):575-82. doi: 10.1007/s00394-013-0566-y. Epub 2013 Jul 30.


Purpose: The combination of resveratrol + conjugated linoleic acid (RSV + CLA) did not show the body fat-lowering effect exhibited by these molecules when administered separately. This study aimed to find metabolic explanations for this situation in an experimental model of diet-induced obesity.

Methods: Thirty-six male Wistar rats were divided into four groups: rats treated with saline (control), resveratrol (RSV), conjugated linoleic acid (CLA) and a combination of these molecules (RSV + CLA).

Results: Rats treated with RSV + CLA did not show the reduction in heparin-releasable lipoprotein lipase (HR-LPL) and fatty acid synthase activities observed in RSV group or the increased HSL expression found in RSV and CLA groups. These animals showed reduced sirtuin 1 expression and CLA isomer amounts in adipose tissue. Finally, intracellular Ca(2+) concentration was increased.

Conclusion: The attenuation of the effects induced in adipose tissue triacylglycerol metabolism by RSV and CLA separately, such as the decrease in lipogenesis and fatty acid uptake and the increase in lipolysis, contributes to explain the lack of body fat-lowering effect of the combination RSV + CLA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / chemistry
  • Adipose Tissue / drug effects*
  • Adipose Tissue / metabolism*
  • Animals
  • Calcium / analysis
  • Drug Interactions
  • Fatty Acid Synthases / metabolism
  • Gene Expression
  • Linoleic Acids, Conjugated / administration & dosage*
  • Linoleic Acids, Conjugated / analysis
  • Lipase / genetics
  • Lipase / metabolism
  • Lipoprotein Lipase / genetics
  • Lipoprotein Lipase / metabolism
  • Male
  • Rats
  • Rats, Wistar
  • Resveratrol
  • Sirtuin 1 / analysis
  • Sirtuin 1 / genetics
  • Stilbenes / administration & dosage*
  • Stilbenes / analysis
  • Stilbenes / metabolism
  • Triglycerides / metabolism*


  • Linoleic Acids, Conjugated
  • Stilbenes
  • Triglycerides
  • Fatty Acid Synthases
  • Lipase
  • Lipoprotein Lipase
  • Sirtuin 1
  • Resveratrol
  • Calcium