The transience of transient overexpression

Nat Methods. 2013 Aug;10(8):715-21. doi: 10.1038/nmeth.2534.


Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Gene Dosage
  • Gene Expression Regulation*
  • Genome*
  • Humans
  • Models, Molecular
  • Mutation
  • Protein Engineering / methods
  • Signal Transduction / genetics
  • Transfection / methods*