Transcriptomic profiling in childhood H1N1/09 influenza reveals reduced expression of protein synthesis genes

J Infect Dis. 2013 Nov 15;208(10):1664-8. doi: 10.1093/infdis/jit348. Epub 2013 Jul 29.


We compared the blood RNA transcriptome of children hospitalized with influenza A H1N1/09, respiratory syncytial virus (RSV) or bacterial infection, and healthy controls. Compared to controls, H1N1/09 patients showed increased expression of inflammatory pathway genes and reduced expression of adaptive immune pathway genes. This was validated on an independent cohort. The most significant function distinguishing H1N1/09 patients from controls was protein synthesis, with reduced gene expression. Reduced expression of protein synthesis genes also characterized the H1N1/09 expression profile compared to children with RSV and bacterial infection, suggesting that this is a key component of the pathophysiological response in children hospitalized with H1N1/09 infection.

Keywords: Peptide Chain Initiation; RSV; eIF-2 Kinase; gene expression profiling; influenza; microarray analysis; pediatric; respiratory tract infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Bacterial Infections / genetics
  • Bacterial Infections / immunology
  • Bacterial Infections / metabolism
  • Child
  • Cluster Analysis
  • Gene Expression Profiling*
  • Gene Expression Regulation*
  • Humans
  • Influenza A Virus, H1N1 Subtype*
  • Influenza, Human / genetics*
  • Influenza, Human / immunology
  • Influenza, Human / metabolism
  • Protein Biosynthesis / genetics*
  • Reproducibility of Results
  • Respiratory Syncytial Virus Infections / genetics
  • Respiratory Syncytial Virus Infections / immunology
  • Respiratory Syncytial Virus Infections / metabolism
  • Respiratory Syncytial Virus, Human
  • Signal Transduction