Identification and Characterization of the Genomic Termini and cleavage/packaging Signals of Gallid Herpesvirus Type 2

Avian Dis. 2013 Jun;57(2 Suppl):401-8. doi: 10.1637/10410-100312-Reg.1.

Abstract

Herpesvirus replication within host cells results in concatemeric genomic DNA, which is cleaved into unit-length genomes and packaged into the capsid by a complex of proteins. The sites of cleavage have been identified for many herpesviruses, and conserved signaling sequences involved in cleavage and packaging have been characterized. The cleavage/packaging motifs pac-1, pac-2, and DR1 and two distinct groups of telomeric repeat sequences (static TRS and variable TRS) have been identified. By sequencing the termini of the gallid herpesvirus type 2 (GaHV-2) strain CU-2, two different cleavage sites (classical and aberrant) have been identified. Unlike classical cleavage of human herpesvirus type 1, which occurs within the DR1 site, classical cleavage of the GaHV-2 concatemers occurs 8.5 bp upstream of the DR1 site and results in an S-terminus containing telomeric repeats. Aberrant cleavage occurs the same distance from the DR1 site and generates a telomeric S-terminus but an L-terminus lacking an a sequence. These results are consistent with previous findings in other herpesviruses and should prove useful in the future study and manipulation of the GaHV-2 genome.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Blotting, Southern / veterinary
  • Cells, Cultured
  • Chick Embryo
  • Chickens*
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Viral / genetics*
  • DNA, Viral / metabolism
  • Herpesvirus 2, Gallid / genetics*
  • Herpesvirus 2, Gallid / physiology
  • Marek Disease / virology*
  • Molecular Sequence Data
  • Nucleocapsid / genetics*
  • Nucleocapsid / metabolism
  • Poultry Diseases / virology
  • Repetitive Sequences, Nucleic Acid
  • Sequence Alignment / veterinary
  • Sequence Analysis, DNA / veterinary
  • Terminator Regions, Genetic
  • Virus Replication

Substances

  • DNA, Viral