Surface occupancy plays a major role in cholesterol's condensing effect

Langmuir. 2013 Aug 20;29(33):10303-6. doi: 10.1021/la402263w. Epub 2013 Aug 6.

Abstract

The condensing power of cholesterol and 5α-cholestane has been examined in liposomal membranes made from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Quantitative nearest-neighbor recognition (NNR) analysis and fluorescence measurements using phase-sensitive probe Laurdan have demonstrated that 5α-cholestane exhibits a substantially weaker condensing effect. This fact, in and of itself, provides compelling evidence that cholesterol's condensing effect is critically dependent on having its steroid nucleus at the membrane surface.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 1,2-Dipalmitoylphosphatidylcholine / analogs & derivatives
  • 1,2-Dipalmitoylphosphatidylcholine / chemistry
  • Cholestanones / chemistry
  • Cholesterol / chemistry*
  • Liposomes / chemistry*
  • Magnetic Resonance Spectroscopy
  • Structure-Activity Relationship

Substances

  • Cholestanones
  • Liposomes
  • 1,2-Dipalmitoylphosphatidylcholine
  • colfosceril palmitate
  • Cholesterol