Comparison of the FDA-approved CDC DENV-1-4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping

J Clin Microbiol. 2013 Oct;51(10):3418-20. doi: 10.1128/JCM.01359-13. Epub 2013 Jul 31.

Abstract

Dengue virus (DENV) is the agent of the most common vector-borne disease worldwide. Using 199 clinical samples collected from Nicaragua and Sri Lanka, a laboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinically sensitive than the FDA-approved CDC assay for DENV serotypes 1 to 4 when measured against a composite reference standard, with sensitivities of 97.4% versus 87.1%, respectively.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Dengue / diagnosis*
  • Dengue / virology*
  • Dengue Virus / classification*
  • Dengue Virus / isolation & purification*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Nicaragua
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Serotyping
  • Sri Lanka
  • Virology / methods*