Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Aug;33(4):581-586.
doi: 10.1007/s11596-013-1162-3. Epub 2013 Aug 1.

Silencing endothelin-3 Expression Attenuates the Malignant Behaviors of Human Melanoma Cells by Regulating SPARC Levels

Affiliations

Silencing endothelin-3 Expression Attenuates the Malignant Behaviors of Human Melanoma Cells by Regulating SPARC Levels

Xiang-Jie An et al. J Huazhong Univ Sci Technolog Med Sci. .

Abstract

Endothelin-3 (ET-3) is aberrantly expressed in both metastatic melanoma tissues and cultured melanoma cells. Our previous work showed that ET-3 could promote survival of metastatic melanoma cells via its altered expression. In this study, we investigated the mechanisms responsible for these gene-induced phenotypes in melanoma cells. An ET-3 gene sequence-specific shRNA vector pLVTHM-ET3-RNAi was constructed and transfected into human malignant melanoma cells A375 and MMRU, and the resultant molecular events and cellular changes were examined. As compared with the empty-vector group, cell proliferation was slowed down, and the growth inhibition rates were 38.9% in A375 cells and 38.4% in MMRU cells after transfection. In addition, cell invasion capability was also inhibited, with a reduction of 62.2% in A375 cells and 54.3% in MMRU cells. The percentage of apoptotic cells was found to increase. Meanwhile, in both cell lines, secreted protein acidic and rich in cysteine (SPARC) levels were down-regulated together with inhibition of its upstream signaling molecule, NF-κB. Thus, the current results suggested that down-regulated expression of ET3 attenuates the malignant behaviors of human melanoma cells partially by decreasing the expression of SPARC and NF-κB.

Similar articles

See all similar articles

Cited by 2 articles

References

    1. Cell Mol Biol (Noisy-le-grand). 1999 Jul;45(5):639-51 - PubMed
    1. Oncogene. 2007 Jun 14;26(28):4084-94 - PubMed
    1. J Huazhong Univ Sci Technolog Med Sci. 2007 Oct;27(5):611-3 - PubMed
    1. Nature. 2000 Aug 3;406(6795):536-40 - PubMed
    1. Hum Pathol. 1999 Mar;30(3):339-44 - PubMed

Publication types

LinkOut - more resources

Feedback