Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays

Analyst. 2013 Oct 7;138(19):5584-7. doi: 10.1039/c3an00891f.


Two types of viral nanoparticles were functionalized with target-specific antibodies and multiple copies of an enzymatic reporter (horseradish peroxidase). The particles were successfully integrated into an immunochromatographic assay detecting MS2 bacteriophage, a model for viral pathogens. The sensitivity of the assay was greatly superior to conventional gold nanoparticle lateral flow assays, and results could easily be evaluated, even without advanced lab instruments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Encephalitis Virus, Japanese / chemistry*
  • Encephalitis Virus, Japanese / genetics
  • Horseradish Peroxidase / analysis
  • Humans
  • Levivirus / chemistry*
  • Levivirus / genetics
  • Molecular Sequence Data
  • Nanoparticles / analysis*


  • Horseradish Peroxidase