Effect of Kombucha, a fermented black tea in attenuating oxidative stress mediated tissue damage in alloxan induced diabetic rats

Food Chem Toxicol. 2013 Oct;60:328-40. doi: 10.1016/j.fct.2013.07.051. Epub 2013 Jul 29.

Abstract

Diabetic complications associated with increased oxidative stress can be suppressed by antioxidants. In the present study we investigated the antidiabetic and antioxidant effects of Kombucha (KT), a fermented black tea, in comparison to that of unfermented black tea (BT), in ALX-induced diabetic rats. ALX exposure lowered the body weight and plasma insulin by about 28.12% and 61.34% respectively and elevated blood glucose level and glycated Hb by about 3.79 and 3.73 folds respectively. The oxidative stress related parameters like lipid peroxidation end products (increased by 3.38, 1.7, 1.65, 1.94 folds respectively), protein carbonyl content (increased by 2.5, 2.35, 1.8, 3.26 folds respectively), glutathione content (decreased by 59.8%, 47.27%, 53.69%, 74.03% respectively), antioxidant enzyme activities were also altered in the pancreatic, hepatic, renal and cardiac tissues of diabetic animals. Results showed significant antidiabetic potential of the fermented beverage (150 mg lyophilized extract/kg bw for 14 days) as it effectively restored ALX-induced pathophysiological changes. Moreover, it could ameliorate DNA fragmentation and caspase-3 activation in the pancreatic tissue of diabetic rats. Although unfermented black tea is effective in the above pathophysiology, KT was found to be more efficient. This might be due to the formation of some antioxidant molecules during fermentation period.

Keywords: 3,3′-diaminobenzidine tetrahydrochloride; ALP; ALT; ALX; Antioxidant machineries; BT; BUN; Black tea; CAT; DAB; Diabetes mellitus; GPx; GR; GSH; GSSG; GST; Kombucha tea; MDA; Oxidative stress; ROS; SOD; TBARS; TC; TG; alanine transaminase; alkaline phosphatase; alloxan monohydrate; black tea; blood urea nitrogen; catalase; glutathione; glutathione disulfide; glutathione peroxidase; glutathione reductase; glutathione-S-transferase; malonaldehyde; reactive oxygen species; superoxide dismutase; thiobarbituric acid reactive substance; total cholesterol; triglyceride.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloxan
  • Animals
  • Antioxidants / analysis
  • Antioxidants / pharmacology*
  • Blood Glucose / metabolism
  • Camellia sinensis / chemistry
  • Caspase 3 / metabolism
  • Cholesterol / blood
  • DNA Fragmentation / drug effects
  • Diabetes Mellitus, Experimental / chemically induced
  • Diabetes Mellitus, Experimental / drug therapy*
  • Fermentation
  • Flavonoids / analysis
  • Flavonoids / pharmacology*
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Hemoglobins / metabolism
  • Insulin / blood
  • Kidney / drug effects
  • Kidney / metabolism
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Oxidative Stress / drug effects*
  • Pancreas / drug effects
  • Pancreas / metabolism
  • Protein Carbonylation / drug effects
  • Rats
  • Reactive Oxygen Species
  • Superoxide Dismutase / metabolism
  • Tea / chemistry*
  • Triglycerides / blood

Substances

  • Antioxidants
  • Blood Glucose
  • Flavonoids
  • Hemoglobins
  • Insulin
  • Reactive Oxygen Species
  • Tea
  • Triglycerides
  • Alloxan
  • Cholesterol
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Casp3 protein, rat
  • Caspase 3
  • Glutathione