Dynamin phosphorylation controls optimization of endocytosis for brief action potential bursts

Elife. 2013 Jul 30:2:e00845. doi: 10.7554/eLife.00845.


Modulation of synaptic vesicle retrieval is considered to be potentially important in steady-state synaptic performance. Here we show that at physiological temperature endocytosis kinetics at hippocampal and cortical nerve terminals show a bi-phasic dependence on electrical activity. Endocytosis accelerates for the first 15-25 APs during bursts of action potential firing, after which it slows with increasing burst length creating an optimum stimulus for this kinetic parameter. We show that activity-dependent acceleration is only prominent at physiological temperature and that the mechanism of this modulation is based on the dephosphorylation of dynamin 1. Nerve terminals in which dynamin 1 and 3 have been replaced with dynamin 1 harboring dephospho- or phospho-mimetic mutations in the proline-rich domain eliminate the acceleration phase by either setting endocytosis at an accelerated state or a decelerated state, respectively. DOI:http://dx.doi.org/10.7554/eLife.00845.001.

Keywords: Mouse; Rat; dynamin; endocytosis; phosphorylation; synaptic vesicle.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials*
  • Calcium / metabolism
  • Dynamins / metabolism*
  • Endocytosis*
  • Hippocampus / metabolism
  • Hippocampus / physiology
  • Humans
  • Kinetics
  • Phosphorylation
  • Temperature


  • Dynamins
  • Calcium