Chemical properties of a polysaccharide purified from solid-state fermentation of Auricularia auricular and its biological activity as a hypolipidemic agent

J Food Sci. 2013 Sep;78(9):H1470-5. doi: 10.1111/1750-3841.12226. Epub 2013 Aug 1.


A water-soluble crude polysaccharide was extracted by hot water from Auricularia auricular mycelium grown under solid-state fermentation (SSF). The crude polysaccharide was purified by DEAE Sephadex A-50 and Sephadex G-200 chromatography. Fourier transform infrared spectroscopy and nuclear magnetic resonance ((1) H NMR) spectroscopy were used to investigate the structure of the purified A. auricular polysaccharide (AAP-I) and revealed that it is α-glycosidically linked. After 14 and 28 days of AAP-I orally administered, the AAP-I significantly decreased the levels of total cholesterol, triglyceride, and low-density lipoprotein cholesterol in mice in which hyperlipidemia had been induced by a high fat diet (P < 0.05). The results revealed that AAP-I from SSF of A. auricular mycelium possesses potent hypolipidemic properties. The polysaccharide may be useful as a functional food additive and a hypolipidemic agent.

Keywords: Auricularia auricula; chemical properties; hypolipidemic; polysaccharide; solid-state fermentation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Basidiomycota / metabolism*
  • Cholesterol / blood
  • Dextrans / chemistry
  • Diet, High-Fat
  • Fermentation*
  • Hyperlipidemias / drug therapy
  • Hypolipidemic Agents / pharmacology*
  • Magnetic Resonance Spectroscopy
  • Male
  • Mice
  • Molecular Weight
  • Mycelium / metabolism
  • Polysaccharides / pharmacology*
  • Spectroscopy, Fourier Transform Infrared
  • Triglycerides / blood


  • Dextrans
  • Hypolipidemic Agents
  • Polysaccharides
  • Triglycerides
  • sephadex
  • Cholesterol