DNA methylation profiles provide a viable index for porcine pluripotent stem cells

Genesis. 2013 Nov;51(11):763-76. doi: 10.1002/dvg.22423. Epub 2013 Aug 30.


Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor-intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)-specific hypomethylated loci (EShypo-T-DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso-4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso-4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso-622-14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum-free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs.

Keywords: epigenetics; induced pluripotent stem cells; translational research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst Inner Cell Mass / metabolism
  • Cell Line
  • Chimera
  • DNA Methylation*
  • Embryo, Mammalian
  • Embryonic Stem Cells / metabolism*
  • Gene Expression
  • Genes
  • Genetic Loci*
  • Induced Pluripotent Stem Cells / metabolism*
  • Mice
  • Swine / embryology*
  • Swine, Miniature / embryology*
  • Translational Research, Biomedical