Assaying hematopoiesis using zebrafish

Blood Cells Mol Dis. 2013 Dec;51(4):271-6. doi: 10.1016/j.bcmd.2013.07.009. Epub 2013 Aug 2.


The zebrafish has become a commonly used model for studying hematopoiesis as a result of its unique attributes. Zebrafish are highly suitable for large-scale genetic and chemical screens compared to other vertebrate systems. It is now possible to analyze hematopoietic lineages in zebrafish and validate cell function via transplantation assays. Here, we review advancements over the past decade in forward genetic screens, chemical screens, fluorescence-activated cell sorting analysis, and transplantation assays. Integrating these approaches enables new chemical and genetic screens that assay cell function within the hematopoietic system. Studies in zebrafish will continue to contribute and expand our knowledge about hematopoiesis, and develop novel treatments for clinical applications.

Keywords: AGM; ALM; BM; CHT; ENU; FACS; FSC; GVHD; HCT; Hematopoiesis; ICM; MHC; PAF; PG; PLM; Pol II Associated Factor; SSC; Screens; Stem cell; TGFβ; TIF1γ; Transplantation; WKM; Zebrafish; anterior lateral mesoderm; aorta–gonad–mesonephros; bone marrow; caudal hematopoietic tissue; ethylnitrosourea; fluorescence-activated cell sorting; forward light scatter; graft-versus-host-disease; hCB; hematopoietic cellular transplantation; hours post fertilization; hpf; human cord blood; intermediate cell mass; major histocompatibility complex; posterior lateral mesoderm; prostaglandin; side light scatter; transcription intermediary factor1γ; transforming growth factor beta; whole kidney marrow.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Lineage
  • Hematopoiesis / physiology*
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Immunophenotyping
  • Phenotype
  • Zebrafish / embryology*
  • Zebrafish / physiology*