In vitro antimicrobial properties of silver-polysaccharide coatings on porous fiber-reinforced composites for bone implants

J Mater Sci Mater Med. 2013 Dec;24(12):2775-85. doi: 10.1007/s10856-013-5022-2. Epub 2013 Aug 7.

Abstract

Biostable fiber-reinforced composite (FRC) implants prepared from bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate resin reinforced with E-glass fibers have been successfully used in cranial reconstructions in 15 patients. Recently, porous FRC structures were suggested as potential implant materials. Compared with smooth surface, porous surface allows implant incorporation via bone ingrowth, but is also a subject to bacterial attachment. Non-cytotoxic silver-polysaccharide nanocomposite coatings may provide a way to decrease the risk of bacterial contamination of porous FRC structures. This study is focused on the in vitro characterization of the effect porosity on the antimicrobial efficiency of the coatings against Staphylococcus aureus and Pseudomonas aeruginosa by a series of microbiological tests (initial adhesion, antimicrobial efficacy, and biofilm formation). Characterization included confocal laser scanning microscopy and scanning electron microscopy. The effect of porosity on the initial attachment of S. aureus was pronounced, but in the case of P. aeruginosa the effect was negligible. There were no significant effects of the coatings on the initial bacterial attachment. In the antimicrobial efficacy test, the coatings were potent against both strains regardless of the sample morphology. In the biofilm tests, there were no clear effects either of morphology or of the coating. Further coating development is foreseen to achieve a longer-term antimicrobial effect to inhibiting bacterial implant colonization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents / chemistry*
  • Bacterial Adhesion / drug effects
  • Benzhydryl Compounds / chemistry
  • Biofilms
  • Bone and Bones
  • Chitosan / analogs & derivatives
  • Chitosan / chemistry*
  • Composite Resins
  • Lactose / analogs & derivatives
  • Lactose / chemistry
  • Methacrylates / chemistry
  • Microbial Sensitivity Tests
  • Polyethylene Glycols / chemistry
  • Polymethacrylic Acids / chemistry
  • Polysaccharides / chemistry*
  • Porosity
  • Prostheses and Implants
  • Pseudomonas aeruginosa
  • Silver / chemistry*
  • Staphylococcus aureus

Substances

  • Anti-Infective Agents
  • Benzhydryl Compounds
  • Composite Resins
  • Methacrylates
  • Polymethacrylic Acids
  • Polysaccharides
  • triethylene glycol dimethacrylate
  • Silver
  • Polyethylene Glycols
  • Chitosan
  • 2,2-di(4-methacryloxyphenyl)propane
  • Lactose