Development of gateway binary vectors R4L1pGWB possessing the bialaphos resistance gene (bar) and the tunicamycin resistance gene as markers for promoter analysis in plants

Biosci Biotechnol Biochem. 2013;77(8):1795-7. doi: 10.1271/bbb.130405. Epub 2013 Aug 7.

Abstract

We have developed a new series of R4L1 Gateway binary vectors (R4L1pGWB), which carry the bialaphos resistance gene (bar) or the UDP-N-acetylglucosamine:dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as selection markers that confer BASTA® and tunicamycin resistance on plants respectively. R4L1pGWBs have an attR4-attL1-reporter and can accept an attL4-promoter-attR1 entry clone for easy construction of an attB4-promoter-attB1-reporter clone. The new R4L1pGWBs facilitate promoter:reporter analysis in pre-existing transgenic plants that are resistant to kanamycin or hygromycin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biomarkers
  • Cinnamates / pharmacology
  • Drug Resistance, Microbial / genetics*
  • Genetic Vectors*
  • Hygromycin B / analogs & derivatives
  • Hygromycin B / pharmacology
  • Kanamycin / pharmacology
  • Organophosphorus Compounds / metabolism
  • Plants, Genetically Modified / genetics*
  • Promoter Regions, Genetic
  • Tunicamycin / genetics
  • Tunicamycin / metabolism
  • Uridine Diphosphate Sugars / genetics*

Substances

  • Biomarkers
  • Cinnamates
  • Organophosphorus Compounds
  • Uridine Diphosphate Sugars
  • uridine diphosphate N-acetyllactosamine
  • Tunicamycin
  • bialaphos
  • Hygromycin B
  • hygromycin A
  • Kanamycin