Abstract
We have developed a new series of R4L1 Gateway binary vectors (R4L1pGWB), which carry the bialaphos resistance gene (bar) or the UDP-N-acetylglucosamine:dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as selection markers that confer BASTA® and tunicamycin resistance on plants respectively. R4L1pGWBs have an attR4-attL1-reporter and can accept an attL4-promoter-attR1 entry clone for easy construction of an attB4-promoter-attB1-reporter clone. The new R4L1pGWBs facilitate promoter:reporter analysis in pre-existing transgenic plants that are resistant to kanamycin or hygromycin.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Biomarkers
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Cinnamates / pharmacology
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Drug Resistance, Microbial / genetics*
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Genetic Vectors*
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Hygromycin B / analogs & derivatives
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Hygromycin B / pharmacology
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Kanamycin / pharmacology
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Organophosphorus Compounds / metabolism
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Plants, Genetically Modified / genetics*
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Promoter Regions, Genetic
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Tunicamycin / genetics
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Tunicamycin / metabolism
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Uridine Diphosphate Sugars / genetics*
Substances
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Biomarkers
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Cinnamates
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Organophosphorus Compounds
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Uridine Diphosphate Sugars
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uridine diphosphate N-acetyllactosamine
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Tunicamycin
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bialaphos
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Hygromycin B
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hygromycin A
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Kanamycin