Aging Studies in Drosophila Melanogaster

Methods Mol Biol. 2013;1048:77-93. doi: 10.1007/978-1-62703-556-9_7.


Drosophila is a genetically tractable system ideal for investigating the mechanisms of aging and developing interventions for promoting healthy aging. Here we describe methods commonly used in Drosophila aging research. These include basic approaches for preparation of diets and measurements of lifespan, food intake, and reproductive output. We also describe some commonly used assays to measure changes in physiological and behavioral functions of Drosophila in aging, such as stress resistance and locomotor activity.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Aging / physiology*
  • Animals
  • Caloric Restriction
  • Diet
  • Drosophila Proteins / genetics
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / physiology*
  • Eating
  • Longevity / genetics*
  • Motor Activity / physiology
  • Oxidative Stress / physiology
  • Receptors, G-Protein-Coupled / genetics
  • Reproduction
  • Stress, Physiological / physiology


  • Drosophila Proteins
  • Receptors, G-Protein-Coupled
  • mth protein, Drosophila