Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

Nat Biotechnol. 2013 Aug;31(8):691-3. doi: 10.1038/nbt.2655.
No abstract available

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / genetics
  • Bacterial Proteins / genetics*
  • Base Sequence
  • CRISPR-Associated Protein 9
  • CRISPR-Associated Proteins / genetics*
  • CRISPR-Cas Systems / genetics*
  • Endonucleases / genetics*
  • Gene Editing / methods*
  • Gene Targeting
  • Genome, Plant
  • Homologous Recombination
  • Mutagenesis, Site-Directed / methods*
  • Mutation Rate
  • RNA, Guide / genetics*
  • Tobacco / genetics*

Substances

  • Bacterial Proteins
  • CRISPR-Associated Proteins
  • RNA, Guide
  • CRISPR-Associated Protein 9
  • Cas9 endonuclease Streptococcus pyogenes
  • Endonucleases