Putative prognostic epithelial-to-mesenchymal transition biomarkers for aggressive prostate cancer

Exp Mol Pathol. 2013 Oct;95(2):220-6. doi: 10.1016/j.yexmp.2013.07.010. Epub 2013 Aug 6.

Abstract

Prostate cancer is the second most frequently diagnosed cancer worldwide and is the sixth leading cause of cancer deaths in men, yet it varies greatly in its aggressiveness. Currently, it is not possible to adequately differentiate between patients whose tumors will remain indolent and those patients whose disease will progress, resulting in unnecessary aggressive treatment. Consequently, there is an urgent need to identify markers of prostate cancer progression, invasiveness and metastasis to more accurately predict prognosis. The aim of this study was to assess the ability of key epithelial-to-mesenchymal transition molecules in identifying prostate cancer patients who are likely to develop aggressive tumors. Using 215 archival patient tissue samples, immunohistochemistry was applied to examine the expression and sub-cellular localization of E-Cadherin, Snail, Slug, Twist, Vimentin, BMP-2 and BMP-7. Of the seven markers assessed, a significantly increased expression of Snail protein was observed within the nucleus of prostate cancer cells and was strongly associated with increasing Gleason score and clinical stage. In addition, loss of E-Cadherin expression at the cellular membrane of prostate cancer cells was also significantly associated with increasing Gleason score, clinical stage, and additionally, a reduction in survival.

Keywords: Clinical stage; E-Cadherin; Epithelial-to-mesenchyme transition; Gleason score; Prostate cancer; Snail.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / analysis*
  • Cadherins / analysis
  • Cadherins / biosynthesis
  • Epithelial-Mesenchymal Transition*
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Neoplasm Grading
  • Prognosis
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology*
  • Snail Family Transcription Factors
  • Transcription Factors / analysis
  • Transcription Factors / biosynthesis

Substances

  • Biomarkers, Tumor
  • Cadherins
  • SNAI1 protein, human
  • Snail Family Transcription Factors
  • Transcription Factors