Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima

Arch Pharm Res. 2014 Jun;37(6):706-12. doi: 10.1007/s12272-013-0230-3. Epub 2013 Aug 10.

Abstract

Anti-inflammatory transcriptional effects of nineteen compounds (1-19) from the soft coral Sinularia maxima were evaluated using NF-κB luciferase and reverse transcriptase polymerase chain reaction. Compounds 1, 2, 4, 8, 15, 17, and 18 significantly inhibited TNFα-induced NF-κB transcriptional activity in HepG2 cells in a dose-dependent manner, with IC50 values ranging from 15.81 ± 2.29 to 29.10 ± 1.54 μM. Furthermore, the transcriptional inhibitory function of these compounds was confirmed by a decrease in intercellular adhesion molecule-1 and inducible nitric oxide synthase gene expression levels in HepG2 cells. These results provide a scientific rationale for the use of the soft coral S. maxima warrant further studies to develop new agents for the prevention and treatment of inflammatory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthozoa*
  • Diterpenes / chemistry*
  • Diterpenes / isolation & purification
  • Diterpenes / toxicity
  • Hep G2 Cells
  • Humans
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / metabolism*
  • Transcriptional Activation / drug effects*
  • Transcriptional Activation / physiology*

Substances

  • Diterpenes
  • NF-kappa B