Culturing and expansion of "clinical grade" precursors cells from the fetal human central nervous system

Methods Mol Biol. 2013;1059:65-77. doi: 10.1007/978-1-62703-574-3_6.


NSCs have been demonstrated to be very useful in grafts into the mammalian central nervous system to investigate the exploitation of NSC for the therapy of neurodegenerative disorders in animal models of neurodegenerative diseases. To push cell therapy in CNS on stage of clinical application, it is necessary to establish a continuous and standardized, clinical grade (i.e., produced following the good manufacturing practice guidelines) human neural stem cell lines. In this chapter, we illustrate some of the protocols routinely used into our GMP cell bank for the production of "clinical grade" human neural stem cell lines.

MeSH terms

  • Animals
  • Cell Proliferation
  • Central Nervous System / cytology
  • Cryopreservation
  • Culture Media
  • Fetus / cytology
  • Humans
  • Karyotyping
  • Mice
  • Mice, Nude
  • Neural Stem Cells / physiology*
  • Neural Stem Cells / transplantation
  • Primary Cell Culture / standards*
  • Spheroids, Cellular / physiology
  • Stem Cell Niche
  • Tissue Banks


  • Culture Media