Importance of Toxin A, Toxin B, and CDT in Virulence of an Epidemic Clostridium Difficile Strain

J Infect Dis. 2014 Jan 1;209(1):83-6. doi: 10.1093/infdis/jit426. Epub 2013 Aug 9.

Abstract

Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.

Keywords: CDT; Clostridium difficile infection; TcdA; TcdB; pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP Ribose Transferases / genetics
  • ADP Ribose Transferases / physiology*
  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology*
  • Bacterial Toxins / genetics
  • Cell Death
  • Clostridium Infections / microbiology*
  • Clostridium Infections / pathology
  • Clostridium difficile / genetics
  • Clostridium difficile / pathogenicity*
  • Cricetinae
  • Enterotoxins / genetics
  • Enterotoxins / physiology*
  • Female
  • HT29 Cells
  • Humans
  • Mesocricetus
  • Virulence / genetics
  • Virulence / physiology

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Enterotoxins
  • tcdA protein, Clostridium difficile
  • toxB protein, Clostridium difficile
  • ADP Ribose Transferases
  • actin-specific ADP-ribosyltransferase, Clostridium