Identification of a FXIIIA variant in human neuroblastoma cell lines

Martina lannaccone; Gaia Giuberti; Giulia De Vivo; Michele Caraglia; Vittorio Gentile

Identification of a FXIIIA variant in human neuroblastoma cell lines

Int J Biochem Mol Biol. 2013 Jul 29;4(2):102-7. Print 2013.

Authors

Martina lannaccone¹, Gaia Giuberti, Giulia De Vivo, Michele Caraglia, Vittorio Gentile

Affiliation

¹ Department of Biochemistry, Biophysics and General Pathology, Second University of Naples 80138 Naples, Italy.

PMID: 23936741
PMCID: PMC3729257

Abstract

FXIII is a transglutaminase consisting of two catalytic (FXIIIA) and two non-catalytic subunits (FXIIIB) in plasma, where this enzyme is responsible for stabilizing fibrin clots. Although possible functions of intracellular FXIIIA have been proposed, these remain to be established. We show that a 40 kDa protein species of FXIIIA is present in the human neuroblastoma cell lines SH-SY5Y and LAN5. These data reveal the presence of a new uncharacterised variant of FXIIIA, possibly due to an alternative splicing, in nervous cells.

Keywords: CNS; Transglutaminases; cellular factor XIII; post-translational modifications of proteins.