iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis

Biomarkers. 2013 Nov;18(7):565-72. doi: 10.3109/1354750X.2013.810667. Epub 2013 Aug 12.

Abstract

Objective: We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA).

Methods: Articular cartilage was collected from patients with OA or femoral neck fracture for the control group. Molecular variations were detected by the iTRAQ method, and quantitative analyses were performed by western blot.

Results: Using the iTRAQ method, we identified 76 proteins with different expression levels in OA patients and the control group. Among these proteins, we selected LECT2 (leukocyte cell-derived chemotaxin-2), BAALC (brain and acute leukemia, cytoplasmic), and PRDX6 (peroxiredoxin-6), which had not been reported as biomarkers for OA.

Conclusions: Use of these proteins in combination with conventional OA biomarkers may better reflect the grade and prognosis of OA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism
  • Cartilage, Articular / metabolism*
  • Case-Control Studies
  • Female
  • Femur Head / metabolism*
  • Humans
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Male
  • Middle Aged
  • Neoplasm Proteins / metabolism
  • Osteoarthritis, Hip / diagnosis
  • Osteoarthritis, Hip / metabolism*
  • Osteoarthritis, Knee / diagnosis
  • Osteoarthritis, Knee / metabolism*
  • Peroxiredoxin VI / metabolism
  • Prognosis
  • Proteome / metabolism*
  • Proteomics

Substances

  • BAALC protein, human
  • Biomarkers
  • Intercellular Signaling Peptides and Proteins
  • LECT2 protein, human
  • Neoplasm Proteins
  • Proteome
  • PRDX6 protein, human
  • Peroxiredoxin VI