Abstract
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. We showed that this approach could be applied in a large population with different genetic backgrounds. UCs are easily accessible and exhibit high reprogramming efficiency, offering advantages over other cell types used for the purpose of iPS generation. Using the approach described in this study, we have generated 93 iPS cell lines from 20 donors with diverse genetic backgrounds. The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Cell Culture Techniques / methods*
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Child
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Child, Preschool
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Female
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Humans
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Induced Pluripotent Stem Cells / cytology*
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Male
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Middle Aged
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Urine / cytology*
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Young Adult
Grants and funding
This work is supported by "Strategic Priority Research Program" of the Chinese Academy of Sciences Grant No. XDA01020202 and XDA01020401 and National Basic Research Program of China, 973 Program of China, 2012CB966503 and 2012CB966802 and National S&T Major Special Project on Major New Drug Innovation, Grant No.2011ZX09102-010, National Natural Science Foundation of China (31200970), D. Pei and G. Pan are supported by the "100 Talents Project" of Chinese Academy of Sciences, China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.