A 12-month randomized double blind controlled trial was conducted among 182 Indonesian postmenopausal women aged 47 to 60 years to determine the effect of 100 mg/day soy isoflavone supplementation on vascular endothelial function such as vascular cell adhesion molecule-1 (VCAM-1), nitric oxide (NO) and malondialdehyde (MDA) as oxidative stress marker. The subjects were randomized to the intervention group receiving tablets consisting of 100 mg soy isoflavones and calcium carbonate 500 mg, and to the control group receiving 500 mg calcium carbonate. The concentrations of VCAM-1, NO and MDA were measured at baseline, and postsupplementation at 6 months and 12 months. After supplementation, the MDA concentrations were significantly lower in the soy isoflavone group compared with the control group (p=0.001). The concentrations of VCAM-1 and NO were not affected (p=0.992 and p=0.759, respectively). In all group the MDA concentration increased compared with baseline concentrations but the relative change of MDA concentrations was significantly lower in the soy isoflavone group compared with the control group. This study demonstrates that supplemental intake of soy isoflavones for 6 months and 12 months had an effect on oxidative stress by decreasing MDA concentration, but did not improve vascular endothelial function.
共182 位，年齡介於47 至60 歲，已停經的印尼婦女，參與為期一年的隨機雙 盲對照試驗。研究目的為評估停經後婦女，每日攝取100 毫克大豆異黃酮補充 劑，對於血管內皮功能，如血管細胞黏附分子-1(VCAM-1)、一氧化氮(NO)， 以及氧化壓力指標-丙二醛(MDA)的影響。受試者被隨機分派至介入組及控制 組，介入組攝取含有100 毫克大豆異黃酮及500 毫克碳酸鈣的錠片，控制組僅 攝取500 毫克碳酸鈣片。於介入前、介入後6 個月、及介入一年，三個時間點 檢測受試者血管細胞黏附分子-1、一氧化氮以及丙二醛濃度。研究結果顯示， 介入組在攝取大豆異黃酮補充劑後，比起控制組，有較低的丙二醛濃度 (p=0.001)，但血管細胞黏附分子-1 及一氧化氮濃度則無顯著差異(p=0.992 與 p=0.759)。兩組的丙二醛濃度皆增加，但介入組的相對改變量顯著低於控 制組。本研究證實，攝取大豆異黃酮補充劑6 個月與12 個月，有助於減少丙 二醛的濃度，藉以降低氧化壓力產生，但無法改善血管內皮功能。