Cloning and sequencing of a human pancreatic tumor mucin cDNA

J Biol Chem. 1990 Sep 5;265(25):15294-9.


A monospecific polyclonal antiserum against deglycosylated human pancreatic tumor mucin was used to select human pancreatic mucin cDNA clones from a lambda gt11 cDNA expression library developed from a human pancreatic tumor cell line. The full-length 4.4-kilobase mucin cDNA sequence included a 72-base pair 5'-untranslated region and a 307-base pair 3'-untranslated region. The predicted amino acid sequence for this cDNA revealed a protein of 122,071 daltons containing 1,255 amino acid residues of which greater than 60% were serine, threonine, proline, alanine, and glycine. Approximately two-thirds of the protein sequence consisted of identical 20-amino acid tandem repeats which were flanked by degenerate tandem repeats and nontandem repeat sequences on both the amino-terminal and carboxyl-terminal ends. The amino acid sequence also contained five putative N-linked glycosylation sites, a putative signal sequence and transmembrane domain, and numerous serine and threonine residues (potential O-linked glycosylation sites) outside and within the tandem repeat position. The cDNA and deduced amino acid sequence of the pancreatic mucin sequence was over 99% homologous with a mucin cDNA sequence derived from breast tumor mucin, even though the native forms of these molecules are quite distinct in size and degree of glycosylation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Neoplasm / genetics*
  • DNA, Neoplasm / isolation & purification
  • Gene Library
  • Humans
  • Immune Sera
  • Molecular Sequence Data
  • Mucins / genetics*
  • Pancreatic Neoplasms / genetics*
  • Protein Biosynthesis
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Repetitive Sequences, Nucleic Acid


  • DNA, Neoplasm
  • Immune Sera
  • Mucins
  • RNA, Neoplasm

Associated data

  • GENBANK/J05582