Abstract
Enamel matrix derivative (EMD), an extract of fetal porcine enamel, and TGF-β can both suppress adipogenic differentiation. However, there have been no studies that functionally link the role of EMD and TGF-β in vitro. Herein, we examined whether TGF-β signaling contributes to EMD-induced suppression of adipogenic differentiation. Adipogenesis was studied with 3T3-L1 preadipocytes in the presence of SB431542, an inhibitor of TGF-βRI kinase activity. SB431542 reversed the inhibitory effect of EMD on adipogenic differentiation, based on Oil Red O staining and mRNA expression of lipid regulated genes. SB431542 also reduced EMD-stimulated expression of connective tissue growth factor (CTGF), an autocrine inhibitor of adipogenic differentiation. Moreover, short interfering (si)RNAs for CTGF partially reversed the EMD-induced suppression of lipid regulated genes. We conclude that the TGF-βRI - CTGF axis is involved in the anti-adipogenic effects of EMD in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3-L1 Cells
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Adipocytes / cytology*
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Adipocytes / drug effects*
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Adipocytes / metabolism
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Adipogenesis / drug effects*
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Adipogenesis / genetics
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Animals
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Benzamides / pharmacology
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Cell Differentiation / drug effects*
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Cell Differentiation / genetics
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Connective Tissue Growth Factor / genetics
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Connective Tissue Growth Factor / metabolism
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Dental Enamel Proteins / pharmacology*
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Dioxoles / pharmacology
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Gene Expression Regulation / drug effects
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Hot Temperature
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Indomethacin / pharmacology
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Mice
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Protein Serine-Threonine Kinases / antagonists & inhibitors
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Protein Serine-Threonine Kinases / metabolism
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Receptor, Transforming Growth Factor-beta Type I
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Receptors, Transforming Growth Factor beta / antagonists & inhibitors
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Receptors, Transforming Growth Factor beta / metabolism
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Rosiglitazone
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Thiazolidinediones / pharmacology
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Transforming Growth Factor beta / pharmacology
Substances
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4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide
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Benzamides
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Dental Enamel Proteins
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Dioxoles
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Receptors, Transforming Growth Factor beta
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Thiazolidinediones
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Transforming Growth Factor beta
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enamel matrix proteins
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Rosiglitazone
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Connective Tissue Growth Factor
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Protein Serine-Threonine Kinases
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Receptor, Transforming Growth Factor-beta Type I
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Indomethacin
Grants and funding
This work was supported in part by Straumann Institute. No additional external funding was received for this study. The authors clearly state that the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.