Implementation of a SPR immunosensor for the simultaneous detection of the 22K and 20K hGH isoforms in human serum samples

Talanta. 2013 Sep 30;114:268-75. doi: 10.1016/j.talanta.2013.04.042. Epub 2013 Apr 30.


We have implemented a Surface Plasmon Resonance (SPR) immunosensor based on a sandwich assay for the simultaneous detection of the two main hGH isoforms, of 22 kDa (22K) and 20 kDa (20K). An oriented-antibody sensor surface specific for both hormone isoforms was assembled by using the biotin-streptavidin system. The immunosensor functionality was checked for the direct detection of the 22K hGH isoform in buffer, which gave high specificity and reproducibility (intra and inter-assay mean coefficients of variation of 8.23% and 9% respectively). The selective determination of the 22K and 20K hGH isoforms in human serum samples in a single assay was possible by using two specific anti-hGH monoclonal antibodies. The detection limit for both hormone isoforms was 0.9 ng mL(-1) and the mean coefficient of variation was below 7.2%. The excellent reproducibility and sensitivity obtained indicate the high performance of this immunosensor for implementing an anti-doping test.

Keywords: Immunosensor; Isoforms; SPR; Serum; Site-directed immobilization; hGH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology
  • Biosensing Techniques*
  • Biotin / chemistry
  • Human Growth Hormone / blood*
  • Human Growth Hormone / immunology
  • Humans
  • Immobilized Proteins / chemistry
  • Male
  • Protein Isoforms / blood
  • Protein Isoforms / immunology
  • Reproducibility of Results
  • Streptavidin / chemistry
  • Surface Plasmon Resonance


  • Antibodies, Monoclonal
  • Immobilized Proteins
  • Protein Isoforms
  • Human Growth Hormone
  • Biotin
  • Streptavidin